The document describes the method of analyzing impurities in octyl-dimethyl-p-aminobenzoic acid using the Agilent 1290 Infinity LC system. The system is capable of operating with long, high resolution columns under conditions of rapid analysis, using low viscosity acetonitrile and higher viscosity methanol as solvent conditions.
The document describes an analysis of octyl-dimethyl-p-aminobenzoic acid (OD-PABA) for the presence of impurities, such as para-aminobenzoic acid (PABA). The study utilizes the Agilent 1290 Infinity LC system equipped with ultra violet/visible Diode Array detection to separate and detect these compounds.
The document demonstrates how to use the Agilent 1290 Infinity LC System with UV/VIS Diode Array detection to analyze octyl-dimethyl-p-aminobenzoic acid for impurities.
The system was used with 3.0 and 2.1 mm id C18 columns, including a 3 mm x 50 mm column and a 2.1 mm x 150 mm column.
The Agilent 1290 Infinity LC system has a pressure limit of approximately 18,000 psi.