The document describes a fast method for the analysis of synthetic antioxidants in vegetable oils. The method uses the Agilent 1290 Infinity LC system equipped with an Agilent ZORBAX Rapid Resolution High Definition (RRHD) column and achieves high throughput in 2 minutes with a backpressure of 1120 bar, which is below the 1200 bar upper limit of the column. Optimization of the mobile phase composition and the temperature are discussed. The figures of merit are illustrated using standard solutions and spiked vegetable oil (sunflower, rapeseed, and olive) extracts. Limits of detection are 1 mg/kg or less in the oil samples. Using a simple methanol extraction, good recovery was obtained for all antioxidants in the oil samples.
This document details a method for the ultrafast analysis of synthetic antioxidants in edible vegetable oils. The method utilizes the Agilent 1290 Infinity LC system equipped with an Agilent ZORBAX Rapid Resolution High Definition (RRHD) column. By optimizing the mobile phase composition and temperature, the analysis time is reduced to approximately 2 minutes with a backpressure of 1120 bar, which is within the column's upper limit of 1200 bar. The approach allows for high-throughput screening of various antioxidants, including propyl gallate, octyl gallate, dodecyl gallate, and ascorbyl palmitate, in sunflower, rapeseed, and olive oil extracts.
| Analysis time | 2 min |
|---|---|
| Backpressure | 1120 bar |
| Column upper limit | 1200 bar |
| Detection limit | 1 mg/kg |
| Column type | ZORBAX RRHD Eclipse Plus C18 |
| Column dimensions | 50 mm L x 2.1 mm id, 1.8 um d |
| Mobile phase A | 0.02% phosphoric acid in water |
| Mobile phase B | Acetonitrile/methanol 50/50 or 75/25 v/v |
The analysis time is reduced to less than 2 minutes.
The backpressure is 1120 bar, which is below the column's 1200 bar upper limit.
The limits of detection are 1 mg/kg or less in the oil samples.
The study used sunflower, rapeseed, and olive oil extracts.