Fast and Ultra-fast Analysis with the Agilent 1200 Series Rapid Resolution LC System Compared to a Conventional Agilent 1100 Series LC System Using Sub 2-µm Particle Columns
This document presents an application note comparing the performance of the Agilent 1200 Series Rapid Resolution LC (RRLC) system against a conventional Agilent 1100 Series LC system. The comparison focuses on the ability to develop faster analytical methods using narrow-bore columns with sub-2-micron particles, which are critical for high-throughput laboratories and LC/MS compatibility.
The study highlights that the Agilent 1200 Series RRLC system offers superior performance due to its wider pressure and temperature ranges, lower delay volumes, and improved noise levels in the diode-array detector. Specifically, the 1200 Series can operate at higher flow rates and temperatures without exceeding pressure limits, resulting in shorter run times and cycle times compared to the 1100 Series.
| Column internal diameter | 2.1 to 4.6 mm |
|---|---|
| Column length | up to 150-mm |
| Column particle size | 1.8-pm |
| Maximum pressure | 600 bar |
| Temperature range | 10 degrees below ambient up to 100 °C |
| Detector data rate | 80 Hz |
The 1200 Series offers wider pressure and temperature ranges, lower delay volumes, and improved noise, allowing for faster methods and higher sample throughput.
Yes, the Agilent 1200 Series RRLC system can be operated with up to 600 bar and up to 100 °C.
The comparison used ZORBAX SB C-18 columns packed with 1.8-pm particles.