Agilent 1200 Series Rapid Resolution LC Technical Note

About this manual

This technical note explains the benefits of using elevated column temperatures (up to 100 °C) with the Agilent 1200 Series Rapid Resolution LC system. It demonstrates that higher temperatures reduce solvent viscosity, allowing for higher flow rates and significantly faster analysis times while maintaining or improving resolution.

What's inside

• Introduction
• Equipment and materials
• Faster analysis time
• Chromatographic conditions
• Resolution
• Peak shape
• Conclusions

Frequently asked questions

What is the maximum column temperature supported by the Agilent 1200 Series Rapid Resolution LC system?

The system's column compartment SL supports temperatures up to 100 °C.

How does increasing the column temperature affect the analysis time for a specific chromatographic run?

Increasing the column temperature from 40 °C to 95 °C shortened the analysis time to 0.4 min.

What flow rate is used when analyzing a Ginseng extract at temperatures of 60 °C, 80 °C, and 100 °C?

A flow rate of 0.5 mL/min is used for the analysis of the Ginseng extract.