This document is an optimization guide for Agilent 1200 Series Rapid Resolution LC and Rapid Resolution LC/MS. It covers the theory of using smaller particles in liquid chromatography, as well as optimization methods for the Agilent 1200 Series RRLC system and Agilent 6000 series Mass Spectrometers instrumentation. The document also includes some application examples.
This guide explains how to optimize Agilent 1200 Series Rapid Resolution LC and LC/MS systems using sub-2-micron particles for faster chromatography and higher resolution. It covers system design, column regeneration, resolution enhancement, sensitivity improvement, and column blockage prevention. The guide also includes optimization for Agilent 6000 series mass spectrometers and application examples for specific compound analyses.
The guide is structured into sections on theory, system optimization, mass spectrometer instrumentation, application examples, and appendices, with detailed explanations of van Deemter equations, particle size effects, and practical implementation steps.
To achieve faster chromatography and higher resolution.
1.8 um particles.