This Technical Note evaluates the precision of retention times for gradient and isocratic applications, and demonstrates that linear and stepwise gradients are delivered with excellent accuracy and precision.
The document presents a technical evaluation of the Agilent 1200 Series Rapid Resolution LC system. It assesses the precision of retention times for both gradient and isocratic applications using standard bore and narrow-bore columns. The study demonstrates that the selected flow is delivered precisely and mobile phases are mixed reliably over the complete gradient range.
The note details experiments conducted with specific modules, including the binary pump SL, high-performance autosampler SL, thermostatted column compartment SL, and diode-array detector SL. It compares standard delay volume configurations (600-800 pL) optimized for resolution against low delay volume configurations (120 pL) suited for speed and LC/MS applications.
The pump can be used up to pressures of 600 bars.
In the standard configuration, the delay volume is between 600 and 800 pL depending on the pressure.
Switching the damper and mixer out of the flow path results in a delay volume of 120 pL.