This Application Note describes the analysis of several degradation products of metoprolol with the Agilent 1120 Compact LC. The preceding method development was done with the Agilent 1200 Series Rapid Resolution LC (RRLC) system. This Application Note starts with the final result of method development, to prove that no further development is necessary. It shows that the Agilent 1120 Compact LC works as a reliable and highly robust instrument for standard LC methodology. The transfer from rapid resolution separation material to conventional columns by the Method Translator Software is shown. The results of system suitability and performance tests prove the reliability and quality of the Agilent 1120 Compact LC system for everyday quality control testing.
The document details a method for analyzing several degradation products of metoprolol tablets. The initial method development was performed using an Agilent 1200 Series Rapid Resolution LC (RRLC) system, and the final results were transferred to the Agilent 1120 Compact LC for routine quality control testing.
The note demonstrates that the transfer from rapid resolution separation material to conventional columns is successful using the Agilent Method Translator Software. System suitability and performance tests confirm the reliability and precision of the Agilent 1120 Compact LC system for everyday quality control applications.
The Agilent Method Translator Software is used to transform parameters from rapid resolution systems to conventional HPLC systems.
The limit for precision of retention times is less than 0.5% RSD.