Key facts
- Brand
- Agilent
- Model
- Protein 80
- Documents
- 5
- Languages
- EN
- Last added
- 2012-07-05
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Agilent Protein 80 Kit Guide
The Agilent Protein 80 Kit is a reagent kit designed for use with the Agilent 2100 Bioanalyzer. It includes Protein Chips, Protein 80 Reagents, and supplies necessary for conducting high-sensitivity protein assays. The kit allows for the analysis of up to 10 samples per chip within a sizing range of 5-80 kDa.
Read onlineAgilent Protein 80 specifications
| Sizing range Protein 80 | 5-80 kDa |
|---|---|
| Sizing range Protein 230 | 14-230 kDa |
| Typical sizing resolution | 10 % |
| Analysis time | 30 minutes |
| Number of samples per chip | 10 samples/chip |
| Sample volume required | 4ul |
| Kit stability | 4 months |
Source: Agilent Series II Protein 80 and 230 Kits Product Leaflet
All Agilent Protein 80 documents by type
How to use the product for one specific task, with worked examples.
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
What is the basic principle for protein sizing?
Protein size determination is calculated via a standard curve from the ladder, which converts measured migration times of unknown proteins to their sizes.
From: Agilent Protein Sizing Quantitation Technical Overview for Protein 80/230 Kits and 2100 Bioanalyzer
How is relative protein concentration calculated?
Relative concentration is calculated by comparing peak areas against an internal upper marker added in a defined ratio to each sample.
From: Agilent Protein Sizing Quantitation Technical Overview for Protein 80/230 Kits and 2100 Bioanalyzer
What is the main advantage of using these kits over SDS-PAGE?
The kits offer improved data reliability with pre-packaged reagents and standardized assays that yield highly reproducible data.
From: Agilent Series II Protein 80 and 230 Kits Product Leaflet
How long does it take to analyze 10 samples?
Sizing and quantitation of 10 samples takes less than 45 minutes, including sample preparation and data analysis.
From: Agilent Series II Protein 80 and 230 Kits Product Leaflet
What is the advantage of using the Agilent Protein 80 kit over conventional SDS-PAGE?
The method is fast, reproducible, robust, and replaces gel electrophoresis, staining, and densitometric analysis.
From: Agilent 2100 Bioanalyzer and Protein 80 Kit Milk Protein Analysis Application Note
Can the system distinguish between cow, goat, and sheep milk?
Yes, the electrophoretic pattern derived from each species shows a significantly different protein pattern.
From: Agilent 2100 Bioanalyzer and Protein 80 Kit Milk Protein Analysis Application Note