Agilent Protein 80 Kit Guide

Agilent Protein 80 User Guide


Agilent Protein 80 Kit Guide - cover page
Brand
Agilent
Document type
User Manual
Language
English
Pages
32
File format
PDF
File size
1.1 MB
Published
06 June, 2012
Updated
30 September, 2023
MD5 checksum
53AA097F15C0A6584D237DCFC61A5145

About this manual

The Agilent Protein 80 Kit is a reagent kit designed for use with the Agilent 2100 Bioanalyzer. It includes Protein Chips, Protein 80 Reagents, and supplies necessary for conducting high-sensitivity protein assays. The kit allows for the analysis of up to 10 samples per chip within a sizing range of 5-80 kDa.

What's inside

  • Equipment Required
  • Setting Up the Assay Equipment
  • Preparing Solutions
  • Loading Samples and Ladder
  • Inserting a Chip
  • Starting the Chip Run
  • Cleaning Electrodes
  • Checking Results

Specifications

Analysis runtime30 minutes
Sizing range5-80 kDa
Number of samples per chip10 samples/chip
Sample volume4ul
Assay Kit stability4 months

Frequently asked questions

What is the typical sizing accuracy of the Protein 80 Assay?

The typical sizing accuracy is 10% CV for Carbonic Anhydrase (CAII) and Beta-Lactoglobulin (BLG).

What is the analysis runtime for a Protein 80 assay?

The analysis runtime is 30 minutes.

How many samples can be analyzed per chip?

Up to 10 samples can be analyzed on a single chip.

What is the required sample volume for the assay?

The required sample volume is 4ul.

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