This application note describes how to use the Agilent 1220 Infinity LC System to analyze amoxicillin and its five impurities.
The document describes a method for analyzing amoxicillin and its degradation products in pharmaceutical drug development. The study utilizes the Agilent 1220 Infinity LC System equipped with an Autosampler, Column Oven, Variable Wavelength Detector, and a ZORBAX SB-Aq column to separate polar substances requiring high water content in the mobile phase.
The analysis was performed using a gradient method at a flow rate of 2 mL/min (525 bar) over seven minutes. The results demonstrate that impurities down to a level of 0.01% can be detected, validating the system's suitability for pharmaceutical quality control laboratories.
| Column dimensions | 150 mm x 4.6 mm |
|---|---|
| Particle size | 1.8 µm |
| Flow rate | 2 mL/min |
| Maximum pressure | >400 bar (525 bar) |
| Analysis time | 7 minutes |
| Detection limit | 0.01% |
| Column type | Agilent ZORBAX SB-Aq |
| Detector wavelength | 229 nm |
Impurities down to a level of 0.01% could be detected under the specified conditions.
The analysis is completed within seven minutes at a flow rate of 2 mL/min.
The Agilent ZORBAX SB-Aq column with 1.8 µm particles is used.