This document is a technical overview of the Agilent 1220 Infi nity LC System. The system can be used for analytical-scale purifi cation of compounds. It is easy to operate and has a flow rate of up to 10 mL/min. The Agilent 1260 Infi nity Analytical-scale Fraction Collector can be used to collect fractions based on time and peak baseline.
This document provides a technical demonstration of analytical-scale purification using the Agilent 1260 Infinity Analytical-scale Fraction Collector. It details how to perform time- and peak-based fraction collection when combined with an Agilent 1220 Infinity LC System.
The text explains two critical prerequisites for high-purity preparative liquid chromatography: accurate delay time determination using the built-in Delay Sensor, and minimized system dispersion via optimized capillaries. It presents experimental results comparing time-based versus peak-based collection methods to illustrate purity recovery.
The delay volume was determined to be 83 µL.