Significant differences in selectivity can be achieved by changing the ZORBAX StableBond phase. Note selectivity and resolution differences for peptides S1 and S2. Peptides S1-S5 are decapeptides that differ in amino acids at two locations, causing only slight differences in hydrophobicity.
This application note demonstrates significant differences in selectivity and resolution for peptides S1 and S2 when using different ZORBAX StableBond phases (C18, C8, and CN). The study utilizes decapeptides (S1-S5) that differ in amino acids at two locations, resulting in only slight differences in hydrophobicity.
| Column Dimensions | 4.6 x 150 mm |
|---|---|
| Column Part Numbers | 883995-902, 883995-906, 883995-905 |
| Mobile Phase A | 0.1% TFA in H2O |
| Mobile Phase B | 0.085% TFA in ACN |
| Flow Rate | 1 mL/min |
| Detection Wavelength | 210 nm |
| Gradient Program | 0-30% B in 30 min |
Significant differences in selectivity can be achieved by changing the ZORBAX StableBond phase.
The part numbers are 883995-902, 883995-906, and 883995-905.