This document introduces the experimental method of separating the recombinant human growth hormone trypsin digestion fragments by using Agilent ZORBAX 300SB-C18, 5µm, 1 mm x 150 mm microbore column.
The document details an HPLC method for separating peptide fragments generated by treating recombinant human growth hormone with trypsin. This approach is designed to achieve sensitive detection when sample amounts are limited.
The separation was performed using reversed-phase HPLC on a microbore column (1 mm ID x 150 mm) packed with Agilent ZORBAX 300SB-C18 stationary phase.
This document describes the separation of peptide fragments generated by treating recombinant human growth hormone with trypsin.
The procedure uses a column that is 1 mm in internal diameter and 150 mm in length.
The flow rate is set to 50 pL per minute.