Agilent 3100 OFFGEL Fractionator Kit Quick Start Guide describes how to use the Agilent 3100 OFFGEL Fractionator to separate proteins or peptides, and recover the separated components in liquid fractions.
This guide provides step-by-step instructions for preparing solutions and samples for protein and peptide fractionation using the Agilent 3100 OFFGEL Fractionator Kit. It includes reagent preparation, sample handling, and troubleshooting. The kit contains IPG strips, frames, electrodes, and reagents for isoelectric focusing. Best practices for sample preparation and equipment setup are detailed to ensure optimal performance.
The guide also covers how to assemble IPG strips, frames, and electrodes, and how to run and retrieve fractions. Safety precautions, such as wearing gloves and eye protection, are emphasized. Sample preparation varies based on strip size (12 cm or 24 cm) and sample type (protein or peptide).
It separates proteins or peptides according to their isoelectric points, recovering them in liquid fractions.
For complex samples such as serum/plasma, cell lysate, and tissue samples, a range of 50 ug to 5 mg is recommended.
The sample volume should be less than or equal to 0.72 mL.
It can be stored at -20°C for four months.