SideStep II SYBR® Green QRT-PCR Master Mix, 2-Step Instruction Manual
The document serves as an instruction manual for the Agilent SideStep II SYBR Green QRT-PCR Master Mix, 2-Step (Catalog #400909). It details the materials provided in the kit, including reagents for cell lysis, cDNA synthesis, and QPCR amplification. The guide outlines storage conditions and lists additional materials required for use.
| Reaction Volume (cDNA synthesis) | 20-μl reaction volume |
|---|---|
| QPCR Reactions Capacity | 400, 25-μl reactions |
| Lysate Samples Capacity | 100 cell lysate samples |
| Storage Temperature (Kit) | -20°C upon receipt |
| Lysate Stability | at least 20 months |
No, the product is for research use only and not for use in diagnostic procedures.
SideStep lysates are suitable for QRT-PCR analysis for at least 20 months when stored at –80°C.
The kit provides reagents for a 20-μl reaction volume.