Agilent Technologies Brilliant II SYBR Green QRT-PCR AffinityScript Master Mix 2-Step INSTRUCTION MANUAL

This document is the instruction manual for Brilliant II SYBR Green QRT-PCR, AffinityScript Master Mix, 2-Step. It describes the features and characteristics of the product.

Brand: Agilent
Document type: User Manual
Language: English
Pages: 24
File format: PDF
File size: 252 KB
Published: 06 June, 2012
Updated: 28 September, 2023
Model: 2-Step
MD5 checksum: 075AB7B0BECE7273E00803216A01354A
PDF: Agilent Technologies Brilliant II SYBR Green QRT-PCR AffinityScript Master Mix 2-Step INSTRUCTION MANUAL PDF

About this manual

The document provides instructions for using the Brilliant II SYBR Green QRT-PCR, AffinityScript Master Mix, a system designed for streamlined two-step QRT-PCR experiments. The kit includes an AffinityScript QPCR cDNA Synthesis Kit and a Brilliant II SYBR Green QPCR Master Mix to generate high-quality cDNA templates from mRNA or total RNA.

What's inside

Materials Provided
Storage Conditions
Additional Materials Required
Introduction
Reverse Transcription with the AffinityScript Kit
QPCR Analysis using the Brilliant II Master Mix
PreProtocol Considerations
RNA Isolation
cDNA Synthesis Reaction Considerations
Troubleshooting

Specifications

Reaction Volume (cDNA synthesis)	20-1 ul
Reaction Volume (QPCR)	25-ul
cDNA length capability	up to 12 kb in length
Protocol time (RT)	just 25 minutes
Nucleotide mix in QPCR master mix	GATC

Frequently asked questions

What additional equipment is required for this kit?

A spectrofluorometric thermal cycler and nuclease-free PCR-grade water are required.

What is the storage condition for the master mix after thawing?

Store the 2x master mix at 4°C after thawing.

How long is full activity guaranteed for the thawed master mix?

Once thawed, full activity is guaranteed for 6 months.

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