The document describes the method of analyzing amitriptyline, fluoxetine, quetiapine, and sertraline using dried blood spots. Blood is spotted onto a blood spotting card and allowed to dry, then samples are taken from the spot for analysis. A new non-cellulose material can also be used for other biological fluids. The use of dried blood spot analysis for drugs is gaining popularity in clinical trials in the pharmaceutical industry.
This document details a method for analyzing amitriptyline, fluoxetine, quetiapine, and sertraline using dried blood spots (DBS) and a new non-cellulose Dried Matrix Spotting (DMS) media. The procedure involves spotting 15 microliters of blood onto a card, drying it, punching a 2-4 mm core, and desorbing the analytes with solvent for LC-MS/MS analysis.
Experimental results demonstrate the method's performance using an Agilent 1290 LC system coupled with an Agilent 6460 mass spectrometer. The study achieved linearity with correlation coefficients better than 0.995 and relative recoveries within 10% of the true value across three concentration levels (0.5, 5.0, and 500 ng/mL).
| Column | Agilent Poroshell 120 EC-C18 3.0 mm x 50 mm |
|---|---|
| Mobile Phase A | 0.1% Aqueous formic acid |
| Runtime | 3:00 minutes |
| Gas temp | 350 C |
| Gas flow | 10 L/min |
| Nebulizing | 20 psi |
The analysis was performed using an Agilent 1290 LC system and an Agilent 6460 mass spectrometer.
The blood spot should be allowed to dry for at least 2 hours.
A 2-4 mm core is punched from the spot for analysis.
No, Bond Elut DMS cards are intended for DMPK/ADME research applications only and should not be used in diagnostic procedures.