This document describes a dried blood spot technology for drug analysis, including methods for collecting, storing, and analyzing blood samples.
This document details a method for analyzing Clozapine, Nortriptyline, Paroxetine, and Zolpidem in dried blood spots (DBS). It introduces the use of Agilent Bond Elut DMS cards as an effective alternative to traditional cellulose materials for storing and preparing biofluid samples for LC-MS/MS analysis.
The note outlines the experimental procedure, including sample preparation, desorption, and the specific LC-MS conditions used with an Agilent 1290 LC and 6460 Mass Spectrometer. Results demonstrate good linearity, recovery, and precision for the four analytes.
| Column | Agilent Pursuit XRs C18, 50 mm x 2.0 mm |
|---|---|
| Flow rate | 200 L/min |
| Run time | 4:00 minutes |
| Gas temp | 350 C |
| Nebulizing | 20 psi |
| Correlation coefficients | better than 0.995 |
The study analyzed Clozapine, Nortriptyline, Paroxetine, and Zolpidem.
The analysis was performed using an Agilent 1290 LC and 6460 Mass Spectrometer.