Key facts
- Brand
- Agilent
- Model
- 4 Channel
- Documents
- 5
- Languages
- EN
- Last added
- 2012-07-05
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Agilent Bravo Automated Liquid Handling Platform: 384-Channel 10 L Tip Accuracy Precision Manual
The Agilent Bravo Automated Liquid Handling Platform is designed to automate routine laboratory processes, increasing sample processing throughput while improving accuracy and precision. The system features nine deck positions that can be configured with heating, cooling, and shaking stations, accommodating various SBS standard microplates.
This document specifically details a technical overview of the platform's performance when used with a 384-Channel ST Disposable Tip Head and 10 µL tips. It describes the testing methodology involving tartrazine/DMSO solutions and UV/Vis spectrophotometry to measure accuracy and precision across a low volume range (0.3 pL to >2 uL).
Agilent 4 Channel specifications
| Tip Volume Range Tested | 0.3 pL to >2 uL |
|---|---|
| Coefficient of Variance (CV) Target | 5% |
| Accuracy Tolerance | +10% of desired volume |
| Deck Positions | Nine |
Source: Agilent Bravo Automated Liquid Handling Platform: 384-Channel 10 L Tip Accuracy Precision Manual
All Agilent 4 Channel documents by type
- Agilent Bravo Automated Liquid Handling Platform: 384-Channel 10 L Tip Accuracy Precision Manual 2 p
- Agilent Bravo Automated Liquid Handling Platform 384-Channel Tip Accuracy and Precision Overview 2 p
- Agilent 384-Channel 30 L Tip Accuracy Precision with Multiple Tip Attachments Manual 2 p
- Agilent Bravo Automated Liquid Handling Platform 384-Channel Tip Accuracy and Precision Overview 2 p
Mounting, wiring and first power-up. Read it before the hardware goes in.
Questions answered in these manuals
What is the accuracy and precision for 0.3 pL dispenses?
For 0.3 pL, the CV is 3.1% and accuracy is +1.4%.
From: Agilent Bravo Automated Liquid Handling Platform: 384-Channel 10 L Tip Accuracy Precision Manual
What are the results for 2.0 pL dispenses?
For 2.0 pL, the CV is 1.3% and accuracy is +1.6%.
From: Agilent Bravo Automated Liquid Handling Platform: 384-Channel 10 L Tip Accuracy Precision Manual
What is the precision of the Bravo with 30 uL tips at 0.5 pL?
The precision is 2.7% CV.
From: Agilent Bravo Automated Liquid Handling Platform 384-Channel Tip Accuracy and Precision Overview
What is the accuracy of the Bravo with 30 uL tips at 5.0 pL?
The accuracy is +1.0%.
From: Agilent Bravo Automated Liquid Handling Platform 384-Channel Tip Accuracy and Precision Overview
Do Agilent 30 pL tips maintain precision after multiple tip attachments?
Yes, precision remains virtually unchanged with CVs of 2% or below for low volume transfers and 1% for high volume transfers after 100 cycles.
From: Agilent 384-Channel 30 L Tip Accuracy Precision with Multiple Tip Attachments Manual
Does accuracy change after repeated tip attachment cycles?
Accuracy drops slightly over 100 cycles, with less than 3% deviation at low volumes and less than 1% at higher volumes.
From: Agilent 384-Channel 30 L Tip Accuracy Precision with Multiple Tip Attachments Manual