This Application Note describes a method for analyzing the leaching of bisphenol A from baby feeding bottles. Bisphenol A is an endocrine disruptor that can mimic the body's own hormones and may have negative health effects.
This document presents a method for quantifying bisphenol A (BPA) and bisphenol F (BPF) extracted from baby feeding bottles. The method utilizes an Agilent 1260 Infinity LC system with a ZORBAX Eclipse Plus C18 column and fluorescence detection. It also details a transfer of this method to an Agilent 1290 Infinity LC system using an ultrahigh performance liquid chromatography (UHPLC) column, which demonstrated improved resolution and sensitivity.
| TCC temperature | 40°C |
|---|---|
| FLD Excitation | 230 nm |
| FLD Emission | 316 nm |
| Flow rate | 0.9 mL/min |
| LOQ for Bisphenol A | 1.06 ng/mL |
| Sample Recovery | 80% |
The limit of quantitation (LOQ) for bisphenol A was found to be 1.06 ng/mL.
During sample recovery studies, 80% recovery was obtained for bisphenol A.