This document introduces two methods for analyzing fumonisin FB1 and FB2 in food and feed samples using the Agilent 1120 Compact LC system and Agilent 6140 Single Quadrupole LC/MS system: simple extraction method (EM) and immunoaffinity column (IAC) extraction method. The results showed that both methods can successfully analyze food and feed samples. The recovery rate of the simple extraction method is higher than that of the immunoaffinity column method. The recovery rate of the EM method is between 62% and 78%, while that of the IAC method is between 42% and 64%. The simple extraction method has high cost-effectiveness, while the immunoaffinity column method has less matrix effects.
The document describes a method for detecting fumonisins (FB1 and FB2) in food and feed samples. It compares two extraction techniques: a simple extraction method (EM) and an immunoaffinity column (IAC) method, analyzing results using the Agilent 1120 Compact LC System coupled to an Agilent 6140 Single Quadrupole LC/MS System.
The study evaluates linearity, recovery rates, and matrix effects. Results indicate that while the EM method offers higher recoveries (62% to 78%) and is more cost-effective, the IAC method produces less matrix effect with lower recovery rates (42% to 64%). The chromatographic method demonstrated high linearity with an R² > 0.9999.
The recoveries from the EM method range from 62% to 78%.
The chromatographic method is linear with an R² greater than 0.9999.
The recoveries from the IAC method range from 42% to 64%.