This is the instruction manual of Brilliant II SYBR® Green QRT-PCR Master Mix Kit, 1-Step
The document provides instructions for using the Brilliant II SYBR Green QRT-PCR Master Mix Kit, catalog number 600825. This kit is designed for in vitro use to perform absolute or relative quantitation of gene expression via a single-step process that combines cDNA synthesis and PCR amplification.
| Reverse Transcriptase Temperature | 50°C |
|---|---|
| Amplicon Size Limit | up to 300 bp |
A spectrofluorometric thermal cycler and nuclease-free PCR-grade water.
The reverse transcriptase performs optimally at a reaction temperature of 50°C.
Sufficient PCR reagents are provided for four hundred, 25-µl reactions.