This document describes the accuracy and precision of 30μL 384-channel tips, which are stable and constant over 100 cycles of tip attachment and detachment. Accuracy and precision are nearly as good on the 100th liquid transfer as they are on the first.
This document presents a technical study on the precision and accuracy of Agilent 384ST 30 pL disposable tips when used with the Agilent Bravo Automated Liquid Handling Platform. The study explores whether tip integrity is maintained over repeated cycles of tip attachment and detachment, which is relevant for applications reusing disposable tips to reduce costs and disposal time.
The results demonstrate that the tips maintain very good accuracy and precision after up to 100 cycles. Precision remains virtually unchanged with Coefficients of Variation (CVs) of 2% or below for low volume transfers and 1% for high volume transfers. Accuracy shows a slight drop over 100 cycles (less than 3% at low volumes and less than 1% at higher volumes), attributed to water adsorption rather than physical changes to the tip seal.
| Tip Volume | 30 pL |
|---|---|
| Platform | Agilent Bravo Automated Liquid Handling Platform |
| Low Volume Dispense | 1 pL |
| High Volume Dispense | 30 pL |
| Precision CV (Low Volume) | 2% and below |
| Precision CV (High Volume) | 1% |
| Accuracy Drop (Low Volume) | <3% |
| Accuracy Drop (High Volume) | <1% |
Yes, precision remains virtually unchanged with CVs of 2% or below for low volume transfers and 1% for high volume transfers after 100 cycles.
Accuracy drops slightly over 100 cycles, with less than 3% deviation at low volumes and less than 1% at higher volumes.
The accuracy shift is likely caused by the adsorption of water into the tips and reservoir, which dilutes the solution, rather than physical changes to the tip.