This document introduces a superficially porous, silica-microsphere packing called Poroshell™, which has excellent kinetic properties and enables stable, high-resolution gradient chromatography of biomacromolecules such as proteins, polypeptides, nucleic acids, and DNA fragments at high speed. Poroshell™ packings can be used with conventional equipment without loss of performance. This technology is useful for rapidly monitoring preparative separations of genetically engineered macromolecules, process control monitoring of fermentation broths or cell-reaction systems, quantitation of polymerase chain reaction fragments, monitoring protein conjugation reactions, and similar applications where very fast, reproducible separations are required.
This document introduces Poroshell technology, a superficially porous silica-micrasphere packing designed for high-velocity separations of biomacromolecules. The innovation allows for stable, high-resolution gradient chromatography of proteins, polypeptides, and nucleic acids at speeds up to 400 times faster than conventional high-performance separations without loss of resolution.
The text details the structure of the Poroshell particle, which features a solid silica core with a thin porous silica shell. It highlights the use of StableBond C18 stationary phase, which is stable at low pH and high temperatures, enabling rapid analysis. The document provides examples of protein separations and DNA fragment analysis, demonstrating significant reductions in runtime and back pressure compared to totally porous particles.
| Particle Type | Superficially porous, silica-micrasphere packing |
|---|---|
| Speed Improvement | Up to 400 times faster than conventional separations |
| Shell Pore Size | 300 Angstroms |
| Stationary Phase | StableBond C18 |
| Operating Temperature Limit | 90 degrees Celsius |
| Compatible Equipment | Conventional equipment |
It enables stable, high-resolution gradient chromatography of biomacromolecules at up to 400-times faster speeds than conventional separations.
Yes, Poroshell packings can be used with conventional equipment without any loss in performance.
The pore size of the outer shell is 300 Angstroms.