This document discusses the competition between speed, resolution, and sensitivity in modern protein laboratories, and introduces ZORBAX Poroshell column technology as a powerful tool for the rapid separation and analysis of proteins. The technology allows for higher mobile phase linear velocities without losing resolution, resulting in shorter run times. The presence of a thin superficially porous layer enables rapid equilibration of slowly diffusing large molecules. The column utilizes proven StableBond technology to maintain stability at low pH and elevated temperature.
In modern protein laboratories, speed and resolution are often competing goals. This document addresses these challenges by demonstrating how ZORBAX Poroshell column technology enables rapid separation and analysis of proteins without losing resolution. The note specifically compares the performance of ZORBAX Poroshell 300SB-C18 columns against totally porous particle columns under varying flow rates.
The text explains that while increasing flow rate is advantageous for small molecules, it typically leads to band broadening in protein separations due to slower diffusion constants. However, the thin superficially porous layer of ZORBAX Poroshell particles allows large molecules to equilibrate quickly, permitting higher linear velocities and significantly shorter run times without significant loss of resolution.
Increasing the flow rate can lead to band broadening because large molecules cannot move into and out of the pores quickly enough as the mobile phase moves faster.
It allows flow rates to be increased to decrease run time without significant peak broadening due to its shortened diffusion path and tolerance of high linear velocity.
Run times are typically five to ten times shorter than those possible with totally porous columns of the same dimensions.