Agilent 5100 Protein 200 HT-2 Assay Antibody Analysis Application Note

About this manual

This document describes a suitability test for the Agilent 5100 Automated Lab-on-a-Chip Platform (ALP) using the Protein 200 HT-2 Assay. The study focuses on analyzing different antibody samples to determine linearity, dynamic range, sizing reproducibility, and quantification limits. The analysis is performed under both reducing and non-reducing conditions to assess the system's performance compared to traditional methods like SDS-PAGE and SEC.

What's inside

• Abstract
• Introduction
• Material and methods
• Results and discussion
• Linearity of antibody quantitation
• Sizing reproducibility
• Limit of quantification (LOQ)
• System robustness

Specifications

Frequently asked questions

What is the linear dynamic range for non-reduced antibody 1?

A good linearity (R² ≥ 0.98) is achieved up to 1000 pg/mL.

What is the linear dynamic range for reduced antibody 2?

A good linear dynamic range is achieved up to 2000 pg/mL.

What is the limit of quantification (LOQ) for antibody 1 in non-reduced form?

The LOQ is 10 pg/mL, determined by a signal to noise greater than 6.

What is the limit of quantification (LOQ) for antibody 2 in reduced form?

The LOQ is 20 pg/mL, determined by a signal to noise greater than 6.