The document describes a method for high-speed separation of analgesics using smaller particles and column dimensions along with high temperature to resolve seven analgesics in less than one minute. The use of high temperature reduces run times. ZORBAX SB-C18 can operate at higher temperatures and lower pH than other commercial reversed phase columns.
This document presents a high-speed liquid chromatography method designed to separate seven specific analgesics in under one minute. The method utilizes high temperature and a specialized column with small particle sizes to achieve rapid resolution without requiring modifications to the instrument. The approach is particularly suitable for pharmaceutical analysis and can be adapted for LC/MS applications by using a volatile buffer instead of an ion-pair mobile phase. Key benefits include reduced analysis time, maintained resolution, and reduced solvent usage.
| Flow Rate | 2 mL/min |
|---|---|
| Injection Volume | 1 µL |
| Column Temperature | 70°C |
| Detector Wavelength | 275 nm |
The method resolves seven analgesics in less than one minute.
Yes, the volatile buffer can be used in place of the ion-pair mobile phase for LC/MS.