This document provides a series of polymerase selection guides, including the characteristics, yield, sensitivity, speed and target length of the polymerases
This document serves as a selection guide for various Agilent DNA polymerases used in PCR applications. It provides a comparative overview of different enzyme types, including standard Taq, high-fidelity proofreading enzymes like PfuUltra and Herculase, and specialized variants designed for difficult targets such as high GC content or dUTP poisoning. The guide details performance metrics such as error rates, reaction speed, and maximum target length for genomic DNA.
| Reaction Volume | 50 µl |
|---|---|
| PfuUltra II Fusion HS Fidelity | 1 error per 2.5 million bp |
| PfuUltra II Fusion HS Speed | 15 sec/kb |
| Herculase II Fusion Fidelity | 1 error per 770,000 bp |
| Paq5000 Fidelity | Equivalent to Taq |
| PfuUltra DNA Polymerase AD Fidelity | 1 error per 2.5 million bp |
| PfuTurbo Cx Hotstart Fidelity | 1 error per 770,000 bp |
| Easy-A PCR Cloning Enzyme Function | Adds 3' -A overhangs |
ArchaeMaxx is designed to overcome dUTP poisoning, and PfuTurbo Cx Hotstart is the only high-fidelity polymerase that will incorporate dUTP.
Herculase II Fusion is the first routine, high-fidelity polymerase for difficult targets including high GC content.
The target length range is 0-19 kb with little-to-no optimization required.
No, Taq DNA Polymerase is no longer available from Agilent.