This document is the Agilent Protein 230 Kit Guide by Agilent Technologies, which describes the features and characteristics of the product.
The document serves as a user manual for the Agilent Protein 230 Kit, detailing the necessary equipment and supplies required for protein analysis. It outlines the setup procedures for both the Chip Priming Station and the Bioanalyzer instrument before beginning the assay.
The guide provides step-by-step protocols for preparing reagents such as Gel-Dye Mix and Denaturing Solution, loading samples onto Protein Chips, running assays, and interpreting results via the 2100 Expert software. It also includes technical specifications regarding sizing ranges, sensitivity, and compatible buffers.
| Analysis runtime | 25 minutes |
|---|---|
| Sizing range | 14-230 kDa |
| Number of samples per chip | 10 samples/chip |
| Sample volume | 4ul |
| Kit stability | 4 months |
The assay covers a sizing range of 14-230 kDa.
Up to 10 samples can be loaded onto a single chip.
The protocol requires a sample volume of 4ul per well.