ZORBAX Eclipse Plus C18 Rapid-Resolution threaded columns are specially designed for higher pressure operation (up to 600 bar) and are packed with a high-performance microparticulate C18 packing for high-speed reverse-phase HPLC. Eclipse Plus C18 columns are designed for superior peak shape with basic compounds and deliver high efficiency and excellent peak shape with all sample types. Eclipse Plus C18 packing is made by first chemically bonding a dense monolayer of dimethyl-n- octadecylsilane stationary phase to a specially prepared, improved ultra-high purity (≥ 99.995% SiO2), ZORBAX Rx-SIL porous silica support. This special ZORBAX silica support (Type B) is designed to reduce or eliminate strong adsorption of basic and highly polar compounds. The bonded-phase packing is then doubly end-capped using proprietary reagents and procedures to obtain maximum deactivation of the silica surface. Eclipse Plus C18 columns can be used for acidic and neutral samples, but are especially suited for separations of basic compounds that produce poor peak shapes on other columns. These columns can be used for a wide range of applications and over a pH range of 2 to 9, accommodating most popular mobile phases. The uniform, spherical Eclipse Plus C18 particles are based on an improved ZORBAX Rx-SIL support that has a nominal surface area of 160 m2/g and a controlled pore size of 95 Å. Columns are loaded to a stable, uniform bed density using a proprietary high-pressure loading device.
This document provides technical specifications and general descriptions for the Agilent ZORBAX Eclipse PAH column. The column features a polymeric C18 bonded phase on an ultra-high purity silica support, specifically designed to provide high shape selectivity for separating polynuclear aromatic hydrocarbons (PAHs). It is tested with a mixture of 16 priority pollutant PAHs and can also be used for other analytes requiring improved shape selectivity.
| Particle Surface Area | > 99.995% SiO2 support, nominal surface area of 160 m²/g |
|---|---|
| Pore Size | 95 A |
| pH Range | 2 to 8 |
| Maximum Operating Pressure (compression fittings) | 400 bar (6,000 psi) |
| Maximum Operating Temperature | 60 °C below pH 6 |
They are designed for the high-efficiency separation of polynuclear aromatic hydrocarbons (PAHs).
No, it is not recommended to use this column below pH 2 or above pH 8.
Long-term storage should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.