This document describes how to use Agilent ZORBAX Eclipse Plus C18 5µm, 3.5µm and 1.8µm columns for simvastatin analysis
Simvastatin is a widely prescribed cholesterol-lowering drug. This document details how to modify the standard USP method for Simvastatin tablet analysis to utilize shorter Agilent ZORBAX Eclipse Plus C18 columns. The modifications allow for significantly reduced analysis times and solvent usage while maintaining required performance.
| Column Length (USP Method) | 250 mm |
|---|---|
| Run Time (USP Method) | 10 min |
| Flow Rate | 1.5 mL/min |
| Column Temperature | 45 °C |
| Detection Wavelength | 238 nm |
| Injection Volume (250 mm column) | 10 uL |
| Injection Volume (100 mm column) | 6 uL |
| Injection Volume (50 mm column) | 2 uL |
The mobile phase is a mixture of 65% acetonitrile and 35% phosphate buffer at pH 4.5.
The original USP method has a 10-minute run time.
All columns are run at pressures under 400 bar.