Eclipse Plus C18 columns are designed for superior peak shape with basic compounds and deliver high efficiency and excellent peak shape with all sample types. Eclipse Plus C18 is especially useful for the separation of acidic, basic, and other highly polar compounds by reverse-phase liquid chromatography. Eclipse Plus C18 packing is made by first chemically bonding a dense monolayer of dimethyl-n-octadecylsilane stationary phase to a specially prepared, an improved ultra-high purity (>99.995% SiO2), ZORBAX Rx-SIL porous silica support. This special silica support (Type B) is designed to reduce or eliminate strong adsorption of basic and highly polar compounds. The bonded-phase packing is then doubly endcapped using proprietary reagents and procedures to obtain maximum deactivation of the silica surface. Eclipse Plus C18 columns can be used for acidic and neutral samples, but are especially suited for separating basic compounds that produce poor peak shapes on other columns. These columns can be used for a wide range of applications and over a pH range of 2–9, accommodating most popular mobile phases. The uniform, spherical, Eclipse Plus C18 particles are based on an improved ZORBAX Rx-SIL support that has a nominal surface area of 160 m²/g and a controlled pore size of 95Å. Columns are loaded to a stable, uniform bed density using a proprietary high-pressure slurry-loading technique to give maximum column efficiency.
This document describes the Agilent ZORBAX Eclipse Plus C18 column, designed to provide superior peak shapes for basic compounds and high efficiency across all sample types. The packing utilizes an improved ultra-high purity (>99.995% SiO2) ZORBAX Rx-SIL porous silica support with a nominal surface area of 160 m²/g and a controlled pore size of 95Å.
| Silica Support Type | ZORBAX Rx-SIL porous silica support (Type B) |
|---|---|
| Surface Area | 160 m²/g |
| Pore Size | 95Å |
| pH Range | 2–9 |
| Maximum Operating Pressure (up to 9.4 mm id) | 400 bar (6000 psi) |
| Maximum Operation Temperature | 60 °C |
The column can be used for a wide range of applications over a pH range of 2–9.
Long term storage should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.
The inlet frit has a nominal porosity of 2 µm.