The document describes an improved method for analyzing opioids, cocaine, and cocaine metabolites from blood using solid phase extraction followed by LC/MS.
This document describes an improved liquid chromatography/mass spectrometry (LC/MS) method for the analysis of opioids, cocaine, and cocaine metabolites from blood samples. The method utilizes solid phase extraction followed by LC/MS with ZORBAX Eclipse Plus C18 columns to achieve excellent peak shape and resolution without the need for derivatization. It was developed at the Washington State Toxicology Laboratory for use with the Agilent MSD SL system.
| Column | ZORBAX Eclipse Plus C18, 4.6 mm x 150 mm, 5 micron |
|---|---|
| Flow rate | 1 mL/min |
| Column temp. | 60°C |
| Instrument | Agilent 1100 LC/MSD SL |
The method uses a ZORBAX Eclipse Plus C18 column.
No, the method allows for a simple extraction without derivatization.