This document describes the use of LC/MS/MS 6410 for the analysis of chloramphenicol, thiamphenicol, and florfenicol in fish samples. The document also mentions that these compounds are banned in the EU and that LC/MS/MS is used for its greater sensitivity and higher selectivity. The method allows for the detection of all three compounds within less than 6 minutes and shows good performance in negative mode.
This document presents an application method for analyzing chloramphenicol, thiamphenicol, and florfenicol in fish samples using an Agilent 6410A triple quadrupole LC/MS system. The method utilizes a simple reversed-phase liquid chromatography (RRLC) approach to separate the compounds within less than 6 minutes, demonstrating high sensitivity in negative mode.
The procedure details sample preparation protocols for honey, shrimp, and chicken, including solid-phase extraction (SPE) steps. It also provides specific MRM settings for the analysis and reports linearity and sensitivity results, showing signal-to-noise ratios for the target compounds at low concentrations.
| Column | Agilent ZORBAX Eclipse Plus, 2.1 mm x 50 mm, 1.8 pm |
|---|---|
| Flow rate | 0.4 mL/min |
| Column compartment temperature | 45 °C |
| Injection volume | 5 pl |
| Source | ESI - |
| Linearity range | 0.5-20 ppb |
The method detects all three compounds within less than 6 minutes.
The signal-to-noise ratio (S/N) is 81 for CAP at 0.5 ppb.