Sulfonamides are drugs used to treat bacterial infections, including pneumonia, meningitis, and tuberculosis. They work by inhibiting bacterial protein synthesis. Sulfonamide residues in animals may be harmful to human health. This study performed a multiresidue analysis of sulfonamide residues in pork using the Agilent 6410A LC/MS/MS. The method can simultaneously detect multiple sulfonamides and has high sensitivity, which can be used for screening, confirmation, and quantification.
The document details a multiresidue analysis method for simultaneously determining various sulfonamide drugs in pork muscle. The study utilizes the Agilent 6410A LC/MS/MS system to achieve greater sensitivity compared to classic methods like HPLC or ELISA, meeting EU maximum residue level requirements.
The existing EU maximum residue level for all drugs of the sulfonamide group is 100 pg/kg in all food-producing species.
The samples were homogenized for 3 minutes with 10 mL acidified methanol.
Using the RRLC method, all 14 compounds can be eluted within 10 minutes.