This application note describes the application of EPA Method 1694 for screening and confirming the presence of 65 pharmaceuticals in water samples using the Agilent G6410A Triple Quadrupole mass spectrometer (QQQ).
This document presents an analytical methodology for screening and confirming the presence of 65 pharmaceuticals and personal care products (PPCPs) in environmental samples. The method utilizes the Agilent G6410A Triple Quadrupole mass spectrometer and was developed according to the guidelines in EPA Method 1694. It employs four distinct chromatographic gradients and LC conditions tailored to the polarity and extraction of different analytes, using both positive and negative ion electrospray ionization.
| Instrument Model | Agilent G6410A Triple Quadrupole mass spectrometer |
|---|---|
| Column Type (Group 1-3) | Agilent ZORBAX Eclipse Plus C18 |
| Column Type (Group 4) | Agilent ZORBAX HILIC Plus |
| Column Dimensions | 2.1 x 100 mm |
| Column Temperature | 25 °C |
| Injection Volume | 15 pL |
| Linearity of Response | Three orders of magnitude (r > 0.99) |
| Number of Analytes | 65 pharmaceuticals |
The method includes 65 analytes, which is a mixture of PPCPs analyzed by a single MRM transition, plus 17 labeled internal standards.
The method uses positive and negative ion electrospray ionization (ESI+ and ESI-), depending on the specific analyte group.
The method uses Agilent C-18 and Hydrophilic Interaction Chromatography (HILIC) columns for all analytes.