Key facts
- Brand
- Agilent
- Model
- High Sensitivity Protein 250
- Documents
- 9
- Languages
- JA · FR · EN
- Last added
- 2012-07-06
Start here
Agilent High Sensitivity Protein 250 Kit User Manual
This manual provides step-by-step instructions for using the Agilent High Sensitivity Protein 250 Kit to analyze proteins via the Agilent 2100 Bioanalyzer. It covers required equipment, labeling protocols, chip setup, and data interpretation. The kit includes labeling reagents, ladder, gel matrix, and chips for sensitive protein quantification.
The guide specifies compatibility with Agilent 2100 Expert software version B.02.06 and higher, and includes safety notices, workflow diagrams, and troubleshooting for labeling and analysis procedures.
Agilent High Sensitivity Protein 250 specifications
| Linear dynamic range | Up to 4 decades |
|---|---|
| Total protein amount for labeling | >1 ng/uL |
| Analysis time | 30 minutes |
Source: Agilent French verion - High Sensitivity Protein 250 kit - 5989-7997FR Manual
All Agilent High Sensitivity Protein 250 documents by type
How to use the product for one specific task, with worked examples.
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
How long does the IP/HSP250 method take to complete?
The total time required for the IP/HSP250 method is approximately 3 hours.
What is the concentration of the GST labeled PTEN protein added to the sample?
The concentration of the GST labeled PTEN protein added to the sample is 100 ng/uL.
What is the minimum amount of protein sample required for analysis?
The kit requires a minimum of only 5 ng of protein samples for analysis.
From: Agilent French verion - High Sensitivity Protein 250 kit - 5989-7997FR Manual
How long does the complete analysis process take?
The entire process from original sample to numerical data takes less than 2 hours.
From: Agilent French verion - High Sensitivity Protein 250 kit - 5989-7997FR Manual
How does the assay handle salt concentration in samples?
The assay is affected by salt concentration; for example, detection limits in PBS are higher than in water.
From: Agilent 2100 Bioanalyzer High Sensitivity Protein 250 Assay Technical Note
What size range of proteins can this assay analyze?
The assay analyzes proteins from 10 to 250 kDa.
From: Agilent 2100 Bioanalyzer High Sensitivity Protein 250 Assay Technical Note