High Sensitivity Protein 250 kit is the first microfluidic assay for protein detection in the low picogram range. It offers 4 orders of magnitude linear dynamic range, allowing quantification of low concentrated impurities next to dominant main peaks in a single run. Therefore, the kit is very well suited to solve typical protein QA/QC questions – in a bioanalyzer-like, fast and reproducible way.
The High Sensitivity Protein 250 kit is a microfluidic assay designed for protein detection in the low picogram range. It offers an extra-wide linear dynamic range of four orders of magnitude, allowing for the quantification of low-concentrated impurities next to dominant main peaks in a single run. The kit is intended to solve typical protein quality assurance and quality control (QA/QC) questions in a fast, reproducible, and automated manner.
| Sizing range | 10-250 kDa |
|---|---|
| Typical sizing resolution | 10 % difference in MW |
| Sensitivity LOD (S/N >3) | 1 pg/uL (labeled BSA in water) |
| Linear dynamic range | Up to 4 orders of magnitude |
| Quantification reproducibility | 20 % CV (BSA) |
| Analysis time | 30 minutes |
| Number of samples per chip | 10 samples/chip |
| Sample volume for labeling reaction | 5 pL |
| Kit stability | 6 months |
The kit offers sensitivity in the picogram range, with a Limit of Detection (LOD) of 1 pg/uL for labeled BSA in water.
The kit allows for the analysis of 10 samples per chip.
Yes, the kit is designed for use with the Agilent 2100 bioanalyzer.
The analysis time is typically 30 minutes after sample preparation.