This document is the instruction manual for Agilent High Sensitivity Protein 250 Labeling Protocol.
The Agilent High Sensitivity Protein 250 Labeling Protocol describes the procedure for labeling proteins with a fluorescent dye using the Agilent High Sensitivity Protein 250 Kit. This document covers the assay principles, required materials, sample prerequisites, and specific instructions for preparing reagents like the Fluorescent Dye in DMSO.
| Protein Size Range | 10 kDa to 250 kDa |
|---|---|
| Sample Volume per Reaction | 5 ul |
| Optimal pH Range | pH 8.0 - 9.0 |
| Compatible Instrument | Agilent 2100 bioanalyzer only |
The optimal pH for labeling is between 8.0 and 9.0.
Store the reconstituted dye solution in the dark at -20°C for up to 6 months.
Components with primary amino or thiol residues, such as DTT and free Aminoacids, cause negative interference.