Agilent High Sensitivity Protein 250 Kit User Manual

About this manual

This manual provides step-by-step instructions for using the Agilent High Sensitivity Protein 250 Kit to analyze proteins via the Agilent 2100 Bioanalyzer. It covers required equipment, labeling protocols, chip setup, and data interpretation. The kit includes labeling reagents, ladder, gel matrix, and chips for sensitive protein quantification.

The guide specifies compatibility with Agilent 2100 Expert software version B.02.06 and higher, and includes safety notices, workflow diagrams, and troubleshooting for labeling and analysis procedures.

What's inside

• Required Equipment for Agilent High Sensitivity Protein 250 Assay
• Agilent High Sensitivity Protein 250 Assay Principles
• General workflow for the High Sensitivity Assay
• Setting up Assay Equipment and Bioanalyzer
• High Sensitivity Labeling Protocol
• Protocol for On-Chip Analysis of Labeled Proteins
• Checking your Agilent High Sensitivity Protein 250 Results
• Compatibility List for the Labeling Reaction
• List of Known Effects from Dilution Buffers

Frequently asked questions

What software version is required for this guide?

This guide is valid for B.02.06 and higher versions of the Agilent 2100 Expert software.

What are the required equipment for the assay?

Required equipment includes the Agilent 2100 Bioanalyzer, chip priming station, 0.5 ml tubes, vortexer, deionized water, microcentrifuge, and 0.5 ml heating block or water bath (95-100°C).