This application note describes a fast HPLC method for the analysis of DNPH derivatized carbonyls found in air samples. The method employs a 1.8-μm StableBond C18 column and a UV detector, and can separate and identify a standard of the major 13 DNPH derivatized carbonyls. An air sample is analyzed in under 10 minutes and found to contain several carbonyls.
The document presents an HPLC method for the separation and identification of carbonyl compounds found in air samples. The technique involves derivatizing carbonyls with 2,4-dinitrophenyl-hydrazine (DNPH) to form colored hydrazones, which are then analyzed using reversed-phase chromatography.
The column has a stated length of 150 mm.
An air sample was analyzed in under 10 minutes.