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- Brand
- HP
- Model
- G1005A
- Industry
- Industrial
- Documents
- 5
- Languages
- EN
- Last added
- 2012-07-05
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Hewlett-Packard G1005A N-terminal Protein Sequencing System Routine 3.1 PVDF Sequencer Methods Technical Note
This document describes the Routine 3.1 PVDF sequencer methods used with the Hewlett-Packard G1005A N-terminal Protein Sequencing System. These methods implement Edman degradation chemistry for analyzing proteins electroblotted to PVDF membranes in the nanomole to low picomole range.
The manual provides detailed step-by-step instructions and volume specifications for three specific methods: Routine 3.1 PVDF (Column), Routine 3.1 PVDF (Converter), and Routine 3.1/3.5 PTH Std. It also includes a Column Prep method to wash the sequencer column prior to sample loading.
HP G1005A specifications
| Applicable Sample Range | nanomole to low picomole range |
|---|---|
| Routine 3.1 PVDF Column Method Time | 45.4 min (2722.1 sec) |
| Routine 3.1 PVDF Cycle 1 Time | 61.2 min (3670.1 sec) |
| Routine 3.1 PVDF Converter Method Time | 33.1 min (1988.1 sec) |
| Routine PTH Std Delivery Volume | 10 pmol/100 |
| Converter Step 2 R4 Delivery Volume | approximately 70 pl (+5 pl) |
All HP G1005A documents by type
Disassembly, diagnostics and part numbers, for repair.
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the purpose of the Routine Cycle 1 method?
It provides an initial sample cleanup and performs a double coupling on the column.
How is the delivery volume of R4 checked?
Copy Steps 7, 8, and 9 to the clipboard, run from the clipboard, remove the conversion flask after Step 9, unscrew it, and measure the delivered volume.
What is the minimum amount of human serum albumin analyzed in this example?
The application brief describes the sequence analysis of 10 pmols of human serum albumin.
From: Hewlett-Packard G1005A N-terminal Protein Sequencing System Application Brief
What is the typical initial yield for pmol amounts using Routine 3.1 chemistry?
Typical initial yields for pmol amounts are approximately 50%.
From: Hewlett-Packard G1005A N-terminal Protein Sequencing System Application Brief
What is the minimum sample amount required for this sequencing method?
The method is designed for N-terminal sequence analysis of peptides at low pmol levels.
From: HP G1005A N-Terminal Protein Sequencing System Application Brief
What repetitive yield was achieved for the 16-amino acid endorphin sample?
The sequence analysis of the 16-amino acid endorphin resulted in an overall repetitive yield of 90.8%.
From: HP G1005A N-Terminal Protein Sequencing System Application Brief