This document describes the N-terminal sequence analysis of peptides at low pmol levels. By using the HP G1005A N-terminal Protein Sequencing System, sequence analysis of representative peptides at low levels can be performed to determine the complete amino acid sequence as much as possible.
This document is an application brief from Hewlett-Packard detailing the N-terminal sequence analysis of peptides at low picomole (pmol) levels. It focuses on the performance of the HP G1005A N-Terminal Protein Sequencing System when using the "Routine 3.5" sequencing method.
The method is designed for N-terminal sequence analysis of peptides at low pmol levels.
The sequence analysis of the 16-amino acid endorphin resulted in an overall repetitive yield of 90.8%.
The 20-amino acid diazepam fragment resulted in an overall repetitive yield of 97.3%.