Hewlett-Packard G1005A N-terminal Protein Sequencing System Application Brief

About this manual

This document is an application brief for the Hewlett-Packard G1005A N-terminal Protein Sequencing System. It details a specific experiment where 10 pmols of human serum albumin (HSA) were analyzed using Routine 3.1 chemistry.

The system utilizes automated Edman chemistry with miniature biphasic columns consisting of reverse-phase and strong anion exchange phases to facilitate sample clean-up and concentration. The brief reports a repetitive yield of 97.1% for the analysis, demonstrating the system's capability for low picomole level protein sequencing.

What's inside

• Abstract
• Introduction
• Features
• Methods
• Results and Discussion

Frequently asked questions

What is the minimum amount of human serum albumin analyzed in this example?

The application brief describes the sequence analysis of 10 pmols of human serum albumin.

What is the typical initial yield for pmol amounts using Routine 3.1 chemistry?

Typical initial yields for pmol amounts are approximately 50%.

How many amino acid residues can be confidently assigned with repetitive yields greater than 95%?

Repetitive yields greater than 95% permit the confident assignment of 40 or more amino acid residues.