Key facts
- Brand
- HP
- Model
- 241 Protein Sequencer (N+C)
- Documents
- 5
- Languages
- EN
- Last added
- 2012-07-05
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HP 241 Protein Sequencer N+C Service Methods Technical Note
This document describes technical methods for N-terminal protein membrane sequencing using the Hewlett-Packard 241 Protein Sequencer. It outlines specific reagents required for C-terminal sequencing versus N-terminal analysis and explains that these methods are suitable for samples in the low nanomole to low picomole range applied onto a HP Zitex strip.
Read onlineHP 241 Protein Sequencer (N+C) specifications
| Sample Range | low nanomole to low picomole range |
|---|---|
| Waste Volume | less than 1 mL/cycle |
| Reagent Source | Hewlett-Packard |
Source: Hewlett-Packard HP 241 Protein Sequencer N+C Service Manual
All HP 241 Protein Sequencer (N+C) documents by type
Disassembly, diagnostics and part numbers, for repair.
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
Which instruments require the use of the TFA bottle cap remover?
Both the HP 241 Protein Sequencing System and the HP G1004B Protein Chemistry Station require the use of this tool.
What is the purpose of the TFA bottle cap remover?
It is a tool designed for safe removal of caps from TFA bottles to prevent accidental contact with the corrosive acid.
What methods are available for switching between sequencing chemistries?
The N-to-C 4.0 method is used when switching from the N-terminal to the C-terminal chemistry, and the C-to-N 4.0 method is used when switching from the C-terminal to the N-terminal chemistry.
From: Hewlett-Packard 241 Protein Sequencer N-to-C and C-to-N Methods Technical Note
What column configuration is required for these methods?
The sequencer column configuration consists of an empty reverse-phase (RP) sample column mated with an empty strong anion exchange (SAX) column.
From: Hewlett-Packard 241 Protein Sequencer N-to-C and C-to-N Methods Technical Note
What is the purpose of the Cycle 1 method?
It provides a longer initial drying time and a pause for proper HPLC column equilibration after switching from C-terminal sequencing.
From: Hewlett-Packard HP 241 Protein Sequencer N+C Service Manual
Which reagents are used for N-terminal sequencing?
Reagents 1R, 2R, 3R, and TH-Std are not utilized; the document focuses on methods using specific coupling agents like DPP-ITC.
From: Hewlett-Packard HP 241 Protein Sequencer N+C Service Manual