A methodology capable of meeting regulatory requirements has been developed for the determination of chloramphenicol in honey and shrimp. Samples of the two foodstuffs are extracted with Isolute HN-M cartridges and analyzed with both the Agilent 1100 LC/MSD Trap (SL) and the Agilent 1100 LC/MSD (SL) quadrupole with negative mode electrospray ionization. Using deuterated internal standard and one simple sample extraction procedure, both instruments provide a limit of detection at or below 0.1 ppb in both shrimp and honey. Chloramphenicol is a broad range antibiotic that has been banned from foods in the European community and the United States at levels greater than 0.1 ppb.
The document details an analytical methodology developed to determine the presence of chloramphenicol in foodstuffs like honey and shrimp at regulatory levels. The method utilizes Isolute HN-M cartridges for sample extraction followed by analysis with Agilent 1100 LC/MSD Trap or LC/MSD quadrupole mass spectrometers using negative mode electrospray ionization.
The study compares the performance of both instruments, noting that while the LC/MSD provides excellent quantitative results, the LC/MSD Trap offers superior full spectrum confirmation and lower detection limits in complex matrices like shrimp due to reduced interference. The document includes detailed sample preparation protocols, chromatographic conditions, and instrument settings required for analysis.
| Limit of Detection (LOD) | 0.1 ppb in shrimp and honey |
|---|---|
| Column Type | Eclipse XDB C18, 4.6 mm x 180 mm, 5 um |
| Flow-rate | 0.9 mL/min |
| Injection Volume | 100 uL |
The method uses the Agilent 1100 LC/MSD Trap and the Agilent 1100 LC/MSD quadrupole mass spectrometer.
The method achieves a limit of detection at or below 0.1 ppb in both shrimp and honey.
Deuterated (d5) CAP internal standard from Cambridge Isotope Laboratories is used.