A relatively rapid and sensitive analytical method for the quantitation of LSD, iso-LSD, and 2-oxo-3-hydroxy-LSD in urine using a single-quadrupole LC/MS system is reported.
The document presents an electrospray LC/MS method developed to analyze LSD, iso-LSD, and the metabolite 2-oxo-3-hydroxy-LSD in urine samples. The method utilizes a single-quadrupole LC/MSD system (Agilent HP 1100) with solid-phase extraction for sample preparation, avoiding the derivatization required by GC/MS methods.
The study validates the method's precision and accuracy against an independently validated GC/MS/MS method. Results show linear calibration curves from 25 to 5000 pg/ml and demonstrate that the metabolite 2-oxo-3-hydroxy-LSD is often present in urine at concentrations significantly higher than the parent drug LSD, extending the detection window.
| Calibration Range | 25-5000 pg/ml |
|---|---|
| Correlation Coefficient | > 0.99 |
| Column Temperature | 25°C |
| Injection Volume | 20 ul |
| Flow Rate | 0.5 ml/min |
No, the use of LC/MS for the analysis of LSD and related compounds does not require derivatization of the analytes.
Detection of the metabolite 2-oxo-3-hydroxy-LSD extends the detection time-window because it is often present in urine at higher concentrations than LSD.