Agilent 1100 LC/MSD Performance-Based Mass Spectral Library Application Note

About this manual

The document explains that universal liquid chromatography/mass spectrometry libraries do not currently exist due to differences in instrument parameters. It introduces a method for obtaining consistent spectra by tuning instruments to fixed ion ratios rather than specific settings, allowing libraries created on different systems to be compatible.

The guide provides detailed procedures for tuning the Agilent 1100 LC/MSD using an ES Tune Mix (part no. G2421A) across low, mid, and high fragmentation energies. It further details how to convert spectra from the ChemStation into NIST format and append them to a library within the NIST MS Search program for data interpretation.

What's inside

• Introduction
• Experimental Setup
• Procedure for tuning 1100 LC/MSD
• Performance-based Criteria
• Adding spectra into NIST Mass Spectral Search Program
• Data Analysis

Frequently asked questions

What tune mixture is recommended for the 1100 LC/MSD?

The Agilent ES Tune Mix (part no. G2421A) was chosen as the tune mixture.

How should the tuning mix be prepared for flow injection analysis?

The tune mix must be diluted 1:1 using a mixture of 50% acetonitrile and 50% water.

What are the fragmentor voltage ranges for low, mid, and high energy?

The ranges are 70-110 V (or 80-180 V) for low energy, 130-170 V (or 210-310 V) for mid energy, and 200-240 V (or 320-400 V) for high energy.