This document describes the N-terminal sequencing methods and the use of PVDF or PTFE materials in the H HP 241 protein sequencer. These methods are suitable for N-terminal sequence analysis of most protein and peptide samples loaded on a Hewlett-Packard PVDF column, with concentrations ranging from low nanomoles to low picomoles. The document also outlines the method for column pre-treatment using a PVDF column.
This document provides technical details on the N-terminal protein membrane 4.0 sequencer methods implemented on the Hewlett-Packard 241 Protein Sequencer. It describes the use of Edman degradation chemistry (coupling, cleavage, and conversion) specifically for analyzing low nanomole to low picomole range protein samples applied onto a HP Zitex strip.
The manual outlines specific sequencing methods including N-Terminal Protein Membrane 4.0, N-Terminal Flask 4.0, PTH-Std 4.0, and Cycle 1 variations. It details the required reagents (R1 through R3, TH-Std), solvent configurations, and precise step-by-step instructions for both column-based and flask-based sequencing cycles.
| Sample Range | low nanomole to low picomole range |
|---|---|
| Waste Volume | less than 1 mL/cycle |
| Column Configuration | empty RP/SAX biphasic column |
Reagents 1R, 2R, 3R, and the TH-Std are specific for C-terminal sequencing.
No, there is no specialized cycle 1 method for the flask.