This document describes the N-terminal protein membrane 4.0 sequencer methods for the HP 241 protein sequencer (N+C). These methods are suitable for N-terminal sequence analysis of most protein samples in the low nanomole to low picomole range.
This document describes technical methods for N-terminal protein membrane sequencing using the Hewlett-Packard 241 Protein Sequencer. It outlines specific reagents required for C-terminal sequencing versus N-terminal analysis and explains that these methods are suitable for samples in the low nanomole to low picomole range applied onto a HP Zitex strip.
| Sample Range | low nanomole to low picomole range |
|---|---|
| Waste Volume (Routine) | less than 1 mL/cycle |
No, these reagents are specific for C-terminal sequencing and are not utilized for the N-terminal protein membrane 4.0 sequencer methods.
No, once the strip is dry it is loaded into an empty RP/SAX biphasic column without any column preparation.